Room-temperature crystallography using a microfluidic protein crystal array device and its application to protein–ligand complex structure analysis
نویسندگان
چکیده
منابع مشابه
Room-temperature serial crystallography using a kinetically optimized microfluidic device for protein crystallization and on-chip X-ray diffraction. Corrigendum
The name of one of the authors in the article by Heymann et al. [(2014), IUCrJ, 1, 349-360] is corrected.[This corrects the article DOI: 10.1107/S2052252514016960.].
متن کاملRoom-temperature serial crystallography using a kinetically optimized microfluidic device for protein crystallization and on-chip X-ray diffraction
An emulsion-based serial crystallographic technology has been developed, in which nanolitre-sized droplets of protein solution are encapsulated in oil and stabilized by surfactant. Once the first crystal in a drop is nucleated, the small volume generates a negative feedback mechanism that lowers the supersaturation. This mechanism is exploited to produce one crystal per drop. Diffraction data a...
متن کاملAccessing protein conformational ensembles using room-temperature X-ray crystallography.
Modern protein crystal structures are based nearly exclusively on X-ray data collected at cryogenic temperatures (generally 100 K). The cooling process is thought to introduce little bias in the functional interpretation of structural results, because cryogenic temperatures minimally perturb the overall protein backbone fold. In contrast, here we show that flash cooling biases previously hidden...
متن کاملThe room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography
Phytochromes are a family of photoreceptors that control light responses of plants, fungi and bacteria. A sequence of structural changes, which is not yet fully understood, leads to activation of an output domain. Time-resolved serial femtosecond crystallography (SFX) can potentially shine light on these conformational changes. Here we report the room temperature crystal structure of the chromo...
متن کاملconstruction and validation of translation metacognitive strategy questionnaire and its application to translation quality
like any other learning activity, translation is a problem solving activity which involves executing parallel cognitive processes. the ability to think about these higher processes, plan, organize, monitor and evaluate the most influential executive cognitive processes is what flavell (1975) called “metacognition” which encompasses raising awareness of mental processes as well as using effectiv...
ذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Chemical Science
سال: 2020
ISSN: 2041-6520,2041-6539
DOI: 10.1039/d0sc02117b